The Biochemistry Laboratory course is designed to accompany Biochemistry (I) and (II) offered in the Dept. The experiments introduced in Biochemistry Laboratory course include the general techniques used in carbohydrate, lipid, protein and nuclei acid research fields. The laboratory course is outlined below:
1. Acid-base titration of amino acid
2. Qualitative determination of carbohydrates
3. Separation of lipids by TLC
4. Protein purification: salting out and dialysis
5. Quantitative determination of protein
6. Determination of specific enzyme activity
7. Polyacrylamide Gel electrophoresis
8. Western blotting analysis
9. ELISA
10. Bacterial cultures
11. Nucleic acid extraction and determination
12. Nucleic acid amplification: PCR
13. Restriction enzyme digestion
14. Agarose gel electrophoresis
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