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Biochemistry Lab

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Laboratory Browse (Rm.5207)                                                                                                                                                                  

 

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Main purpose

The Biochemistry Laboratory is to provide the space and equipment for experiments offered in the lab course, so that the students will be able to employ the theory and principle of Biochemistry learned from textbook to the general techniques used in different research area. In addition to the basic experiments, the techniques used in research and industrial are introduced in the Biochemistry Laboratory course. The curricular purpose of the Biochemistry Laboratory course are to introduce the experimental techniques to students, initiate the research interest of students and train the students to observe carefully and think independently.

 

Course outlines

The Biochemistry Laboratory course is designed to accompany Biochemistry (I) and (II) offered in the Dept. The experiments introduced in Biochemistry Laboratory course include the general techniques used in carbohydrate, lipid, protein and nuclei acid research fields. The laboratory course is outlined below:


1. Acid-base titration of amino acid
2. Qualitative determination of carbohydrates 
3. Separation of lipids by TLC
4. Protein purification: salting out and dialysis 
5. Quantitative determination of protein
6. Determination of specific enzyme activity
7. Polyacrylamide Gel electrophoresis
8. Western blotting analysis 
9. ELISA
10. Bacterial cultures 
11. Nucleic acid extraction and determination 
12. Nucleic acid amplification: PCR
13. Restriction enzyme digestion
14. Agarose gel electrophoresis 
 

Equipment & Facility

 
                             
 

     

     

     

 
 

-20oC freezer

     

4oC cold box

     

8-channel micropipett

     

Perisist pump

 
                             
                             
                             
 

     

     

     

 
 

UV observation box

     

Hybridization oven

     

Spectrophotometer

     

Shaking water bath

 
                             
 

                             
 

     

     

     

 
 

Stir/hot plate

     

Hot sealer

     

Oval plateform shaker

     

Thermal-control high speed centrifuge

 
                             
                             
                             
 

     

     

     

 
 

Dry block

     

Shaking incubator

     

Luminar flow

     

Microwave oven

 
                             
 

                             
 

     

     

     

 
 

Micropipettman

     

Vortex

     

Balance

     

Protein gel electrophoresis apparatus

 
                             
                             
                             
 

     

     

     

 
 

Polymerase chain reaction machine

     

Microplate reader

     

pH meter

     

Bench-top centrifuge

 
                             
 

 

Safety Rules of Laboratory

Safety Rules of Biochemistry Laboratory

 

 

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