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生物化學實驗課程所進行之實驗,涵蓋醣類、脂肪、蛋白質及核酸等方面。
其課程概列如下:
1. Acid-base titration of amino acid
2. Qualitative determination of carbohydrates
3. Separation of lipids by TLC
4. Protein purification: salting out and dialysis
5. Quantitative determination of protein
6. Determination of specific enzyme activity
7. Polyacrylamide Gel electrophoresis
8. Western blotting analysis
9. ELISA
10. Bacterial cultures
11. Nucleic acid extraction and determination
12. Nucleic acid amplification: PCR
13. Restriction enzyme digestion
14. Agarose gel electrophoresis
資料提供﹕葉貞吟老師
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